Gram staining applied to Human spermatozoa: a simple method for studyng chromatin condensation status

Gram staining applied to human spermatozoa from fertile donors is described. The stain revealed populations of Gram-positive and Gram-negative spermatozoa. Data showed a significant and progressive decrease in the percentage of Gram-positive spermatozoa at different times during the chromatin decondensation procedure (SDS-BSA and SDS-EDTA). No significant correlation could be found between Gram staining and other functional tests used for spermatozoa; only the aniline blue staining test showed a poor correlation. Our study demonstrates that normal spermatozoa with regular chromatin condensation appear Gram-positive, while spermatozoa with altered chromatin condensation appear Gram-negative

Gaseous argon time projection chamber with electroluminescence enhanced optical readout

Systematic uncertainties in accelerator oscillation neutrino experiments arise mostly from nuclear models describing neutrino-nucleus interactions. To mitigate these uncertainties, we can study neutrino-nuclei interactions with detectors possessing enhanced hadron detection capabilities at energies below the nuclear Fermi level. Gaseous detectors not only lower the particle detection threshold but also enable the investigation of nuclear effects on various nuclei by allowing for changes in the gas composition. This approach provides valuable insights into the modelling of neutrino-nucleus interactions and significantly reduces associated uncertainties. Here, we discuss the design and first operation of a gaseous argon time projection chamber optically read. The detector operates at atmospheric pressure and features a single stage of electron amplification based on a thick GEM. Here, photons are produced with wavelengths in the vacuum ultraviolet regime. In an optical detector the primary constraint is the light yield. This study explores the possibility of increasing the light yield by applying a low electric field downstream of the ThGEM. In this region, called the electroluminescence gap, electrons propagate and excite the argon atoms, leading to the subsequent emission of photons. This process occurs without any further electron amplification, and it is demonstrated that the total light yield increases up to three times by applying moderate electric fields of the order of 3~kV/cm. Finally, an indirect method is discussed for determining the photon yield per charge gain of a ThGEM, giving a value of 18.3 photons detected per secondary electron

Hypothalamo-pituitary axis and puberty

Puberty is a complex process that culminates in the acquisition of psychophysical maturity and reproductive capacity. This elaborate and fascinating process marks the end of childhood. Behind it lies a complex, genetically mediated neuroendocrine mechanism through which the gonads are activated thanks to the fine balance between central inhibitory and stimulating neuromodulators and hormones with both central and peripheral action. The onset of puberty involves the reactivation of the hypothalamic-pituitary-gonadal (HPG) axis, supported by the initial “kiss” between kisspeptin and the hypothalamic neurons that secrete GnRH (the GnRH “pulse generator”). This pulsatile production of GnRH is followed by a rise in LH and, consequently, in gonadal steroids. The onset of puberty varies naturally between individuals, and especially between males and females, in the latter of whom it is typically earlier. However, pathological variations, namely precocious and delayed puberty, are also possible. This article reviews the scientific literature on the physiological mechanisms of puberty and the main pathophysiological aspects of its onset

Klinefelter syndrome: cardiovascular abnormalities and metabolic disorders

Klinefelter syndrome (KS) is one of the most common genetic causes of male infertility. This condition is associated with much comorbidity and with a lower life expectancy. The aim of this review is to explore more in depth cardiovascular and metabolic disorders associated to KS. KS patients have an increased risk of cerebrovascular disease (standardized mortality ratio, SMR, 2.2; 95% confidence interval, CI, 1.6-3.0), but it is not clear whether the cause of the death is of thrombotic or hemorrhagic nature. Cardiovascular congenital anomalies (SMR, 7.3; 95% CI, 2.4-17.1) and the development of thrombosis or leg ulcers (SMR, 7.9; 95% CI, 2.9-17.2) are also more frequent in these subjects. Moreover, cardiovascular abnormalities may be at least partially reversed by testosterone replacement therapy (TRT). KS patients have also an increased probability of endocrine and/or metabolic disease, especially obesity, metabolic syndrome and type 2 diabetes mellitus. The effects of TRT on these abnormalities are not entirely clear

Coseismic and initial postseismic slip of the 2009 Mw 6.3 l’Aquila earthquake, Italy, from GPS measurements

Here we report the preliminary results of GPS data inversions for coseismic and initial afterslip distributions of the Mw 6.3 2009 April 6 L’Aquila earthquake. Coseismic displacements of continuous and survey-style GPS sites, show that the earthquake ruptured a planar SW-dipping normal fault with ∼0.6 m average slip and an estimated moment of 3.9 × 1018 Nm. Geodetic data agree with the seismological and geological information pointing out the Paganica fault, as the causative structure of the main shock. The position of the hypocentre relative to the coseismic slip distribution supports the seismological evidence of southeastward rupture directivity. These results also point out that the main coseismic asperity probably ended downdip of the Paganica village at a depth of few kilometres in agreement with the small (1–10 cm) observed surface breaks. Time-dependent post-seismic displacements have been modelled with an exponential function. The average value of the estimated characteristic times for near-field sites in the hanging-wall of the fault is 23.9 ± 5.4 d. The comparison between coseismic slip and post-seismic displacements for the first 60 d after the main shock, shows that afterslip occurred at the edges of the main coseismic asperity with a maximum estimated slip of ∼25 cm and an equivalent seismic moment of 6.5 × 1017 Nm. The activation of the Paganica fault, spatially intermediate between the previously recognized main active fault systems, suggests that strain accumulation in the central Apennines may be simultaneously active on distinct parallel fault systems.Published1539–15461.1. TTC - Monitoraggio sismico del territorio nazionale1.9. Rete GPS nazionaleJCR Journalrestricte

Elastic Scattering and Total Cross-Section in p+p reactions measured by the LHC Experiment TOTEM at sqrt(s) = 7 TeV

Proton-proton elastic scattering has been measured by the TOTEM experiment at the CERN Large Hadron Collider at $\sqrt{s} = 7$ TeV in special runs with the Roman Pot detectors placed as close to the outgoing beam as seven times the transverse beam size. The differential cross-section measurements are reported in the |t|-range of 0.36 to 2.5 GeV^2. Extending the range of data to low t values from 0.02 to 0.33 GeV^2,and utilizing the luminosity measurements of CMS, the total proton-proton cross section at sqrt(s) = 7 TeV is measured to be (98.3 +- 0.2(stat) +- 2.8(syst)) mb.Comment: Proceedings of the XLI International Symposium on Multiparticle Dynamics. Accepted for publication in Prog. Theor. Phy

A narrow band neutrino beam with high precision flux measurements

The ENUBET facility is a proposed narrow band neutrino beam where lepton production is monitored at single particle level in the instrumented decay tunnel. This facility addresses simultaneously the two most important challenges for the next generation of cross section experiments: a superior control of the flux and flavor composition at source and a high level of tunability and precision in the selection of the energy of the outcoming neutrinos. We report here the latest results in the development and test of the instrumentation for the decay tunnel. Special emphasis is given to irradiation tests of the photo-sensors performed at INFN-LNL and CERN in 2017 and to the first application of polysiloxane-based scintillators in high energy physics.Comment: Poster presented at NuPhys2017 (London, 20-22 December 2017). 5 pages, 2 figure

Quantum Dissipative Effects and Neutrinos : current constraints and future perspectives

We establish the most stringent experimental constraints coming from recent terrestrial neutrino experiments on quantum mechanical decoherence effects in neutrino systems. Taking a completely phenomenological approach, we probe vacuum oscillations plus quantum decoherence between two neutrino species in the channels $\nu_\mu \to \nu_\tau$, $\nu_\mu \to \nu_e$ and $\nu_e \to \nu_\tau$, admitting that the quantum decoherence parameter $\gamma$ is related to the neutrino energy $E_\nu$ as : $\gamma=\gamma_0 (E_\nu/\text{GeV})^{n}$, with $n=-1,0,1$ and 2. Our bounds are valid for a neutrino mass squared difference compatible with the atmospheric, the solar and, in many cases, the LSND scale. We also qualitatively discuss the perspectives of the future long baseline neutrino experiments to further probe quantum dissipation.Comment: 26 pages, 8 encapsulated postscript figure

First Results from the TOTEM Experiment

The first physics results from the TOTEM experiment are here reported, concerning the measurements of the total, differential elastic, elastic and inelastic pp cross-section at the LHC energy of $\sqrt{s}$ = 7 TeV, obtained using the luminosity measurement from CMS. A preliminary measurement of the forward charged particle $\eta$ distribution is also shown.Comment: Conference Proceeding. MPI@LHC 2010: 2nd International Workshop on Multiple Partonic Interactions at the LHC. Glasgow (UK), 29th of November to the 3rd of December 201